Isolation and Extraction of Total Flavonoids from Epimedium Koreanum Nakai by Supercritical Fluid Extraction

In the present paper is reported the method for the isolation and extraction of total flavonoids of Epimedium Koreanum Nakai by means of supercritical fluid extraction(SFE). By examining pressure, temperature, amounts of modifier and extraction time, the optimized condition of SFE is confirmed as 30 MPa and 60 ℃, with 70% ethanol as the modifier. The samples were statically extracted for 30 min, followed by dynamic extraction for 120 min at a flow rate of 6 mL/min. The quantitative analysis of total flavonoids was performed by UV-Vis spectrophotometry. Compared with the conventional method, the SFE method is more efficient, more rapid and more friendly environmentally.

作 者： LIU Chun-ming ZHAO Xue-liang LIU Zhi-qiang XING Jun-peng   作者單位： LIU Chun-ming(Laboratory of New Drug Research, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China;Department of Chemistry, Changchun Teachers? College, Changchun 130032, P. R. China)

ZHAO Xue-liang(China-Japan Union Hospital, Jilin University, Changchun 130032, P. R. China)

LIU Zhi-qiang,XING Jun-peng(Laboratory of New Drug Research, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China) 

刊 名： 高等學�；瘜W研究（英文版）  ISTIC SCI 英文刊名： CHEMICAL RESEARCH IN CHINESE UNIVERSITIES  年，卷(期)： 2004 20(6)  分類號： O6  關鍵詞： Supercritical fluid extraction(SFE)   Flavonoids   Epimedium Koreanum Naka  

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